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All samples were labeled with N -(2-aminoethyl) rhodamine 6G-amide bis(trifluoroacetate) (R6G) by using the EDC/NHS coupling reaction with a weight ratio of polypeptide to R6G at 20:1 (Fig

10.2337/diabetes.52.1.124 4 De RooijJ.ZwartkruisF.VerheijenM.CoolR.NijmanS.WittinghoferA.et al

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Because several of these compounds are sensitive to heat and light, careful storage is part of getting reproducible results a degraded compound behaves differently from a fresh one

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