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Ficoll-PaqueTM PLUS (Cytiva) was used to sorting PBMCs

When your body isnt getting enough vitamin B12, red blood cells become enlarged making it harder for them to move in your bloodstream

Le Gal, K

Abbreviations 2OBA: 2-Oxobutanoate 3PG: 3-Phosphoglycerate AUC: Area under the receiver operating characteristic (ROC) curve BILAG: British Isles Lupus Assessment Group cAMP: Cyclic AMP db-cAMP: Dibutyryl cAMP DHAP: Dihydroxy-acetone-phosphate DN T: CD3 + CD4 CD8 double-negative T cell E4P: Erythrose 4-phosphate FAS: Fatigue assessment scale FDR: False discovery rate G1P: Glucose 1-phosphate G6P: Glucose 6-phosphate GA3P: Glyceraldehyde 3-phosphate GL6P: Gluconolactone 6-phosphate GPP: Geranyl pyrophosphate GSH: Reduced glutathione GSSG: Oxidized glutathione HCA: Homocysteic acid HCY: Homocysteine HE: Hydroethidine;ROI sensor IAA: Indol-3-acetic acid Kyn: Kynurenine LCMS/MS: Liquid chromatography-tandem mass spectroscopy Met-SO: Methionine sulfoxide mTOR: Mammalian/mechanistic target of rapamycin NAC: N -acetylcysteine O8P: Octulose 8-phosphate OAA: Oxaloacetate PBL: Peripheral blood lymphocytes PBS: Phosphate buffered saline PCA: Principal component analysis PLS-DA: Partial least square-discriminant analysis PI: Propidium iodide R5P: Ribose 5-phosphate RFI: Relative fluorescence intensity ROC: Receiver operating characteristic S7P: Sedoheptulose 7-phosphate SBP: Sedoheptulose 1,7-bisphosphate SLE: Systemic lupus erythematosus SLEDAI: Systemic lupus erythematosus disease activity index SPE: Squared prediction errors T6P: Trehalose 6-phosphate VIP: Variable importance in projection References Aune, T

At least 100 spermatozoa per sample were evaluated to estimate progressive motility