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RNA Extraction and Quantitative Reverse Transcription-Polymerase Chain Reaction (qRT-PCR) Total RNA was extracted from cellular and cerebral samples using TRIzol reagent (Invitrogen, Carlsbad, CA, United States)

A 10 mg vial in 2 mL of bacteriostatic water gives 5 mg/mL, where a 1 mg dose draws 0.2 mL (20 units on a U-100 syringe) and a 0.5 mg dose draws 0.1 mL (10 units)

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