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Description

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Gastroenterology (2016) 150(1):6478

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A Fast Site-directed Mutagenesis Kit (FM111, TransGen Biotech) was used to generate point mutations in UBE2m (R33K, R116K, R157K, R158K, R166K, R169K, and R180K)

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Mass Spectrometric Screening of Solvent Extractables from Strain F3876/87 Bacterial pellets, prepared from F3876/87 liquid cultures (100 mL) by centrifugation, were extracted with 10 mL of MeOH, MeCN, or MeCN/H 2 O (70/30, v/v), respectively, by shaking overnight (15 h) at room temperature

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