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This has been suggested to promote copper deficiency in AD, as astrocyte ApoE4-mediated copper retention could contribute to reduced neuronal copper availability since astrocytes are key to regulating brain copper levels (Scheiber and Dringen, 2013

Next, cells were incubated with 0.01 mg/mL primary antibody and then with secondary VectaFluor anti-rabbit IgG Dylight 488 antibody for 30 min at RT

People who white-knuckle through weeks 6 to 8 without preparation often fail