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They were housed in standard cages, five per cage, with 12/12 hours light-dark cycles at temperature of 23 2 C

Factors influencing this include dosage, adherence to treatment, lifestyle modifications, and baseline body weight

It is cleaved from preproglucagon within the intestine, where it is released by endocrine L-cells of the distal gut (Wettergren et al., 1997)

It occurred gradually over 6 to 12 months, mirroring the gradual loss in reverse

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