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Stimulation with 6-hydroxydopamine (6-OHDA) markedly downregulates nuclear factor erythroid 2related factor 2 (NRF2) expression in PC-12 cells, elevates Fe 2+ and reactive oxygen species (ROS) levels, suppresses glutathione peroxidase 4 (GPX4), promotes both ferroptosis and apoptosis, inhibits cell proliferation, and causes neuronal damage

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Optimal phase II and III detoxification requires the activation of the cellular master switch Nrf2.12 Nrf2 regulates over 500 genes involved in detoxification

Finally, the MethylFlash Global DNA Methylation (5mC) ELISA Easy Kit (colorimetric) was used according to the manufacturers instructions, to assess global levels of 5-methylcytosine (5mC)

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