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Upon receiving the islet shipment, the islets were washed with fresh culture media (CMRL-1066 supplemented with 0.1 g/L L-glutamine, 10% FBS, 10 mM HEPES, 0.2% Sodium Bicarbonate, and 0.1 mg/ml ciprofloxacin), allocated into 48-well plates at a density of 150 Islet Equivalent Quantities (IEQ) per 0.5 ml per well, and cultured at 37 C in a humidified incubator containing 5% CO 2

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