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A direct test of the diathesis-stress model for depression

Motor cortex is isolated from P3 pups of WT-eGFP and and prpTDP-43 A315T -UeGFP mice, and dissociated cells are plated on glass coverslips to establish motor cortex cultures in vitro 32

Front Endocrinol (Lausanne) (2022) 13:1043789

After infected by 48 h, the cells were harvested by centrifugation at 2000 rpm (Thermo Fisher, H12000) for 20 min and kept frozen at 80 C for further usage

Its small molecular size allows it to penetrate intestinal epithelial cells directly, which is why it has attracted research interest as an orally deliverable gut-targeted compound